rabbit anti mmp12 Search Results


mmp-12 polyclonal antibody  (Bioss)
92
Bioss mmp-12 polyclonal antibody
Mmp 12 Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mmp-12 polyclonal antibody/product/Bioss
Average 92 stars, based on 1 article reviews
mmp-12 polyclonal antibody - by Bioz Stars, 2026-03
92/100 stars
  Buy from Supplier
rabbit anti-human mmp12 abx102901  (Abbexa Ltd)
90
Abbexa Ltd rabbit anti-human mmp12 abx102901
MitoSOX quantification of WT and Ripk3 −/− BMDM treated with HMGB1 ( a ) and WT BMDM treated with HMGB1 in the presence or not of Mdivi-1 or MitoTEMPO ( b ). n = 3 . * P < 0.05, *** P < 0.001. ( c ) Relative mRNA expression of Glud1 in WT or Ripk3 −/− BMDM. n = 3 per group . * P < 0.05, *** P < 0.001. ( d ) Representative IF images of dihydroethidium (DHE) staining (red) of aortic sections of mice treated as indicated. Elastin autofluorescence (green, scale bar = 20 µm). ( e ) Heatmap representation of differentially expressed genes in macrophages expressing Ripk3 ( Ripk3 + ) or not ( Ripk3 − ). ( f ) qPCR analysis of <t>Mmp12</t> mRNA in WT or Ripk3 −/− aortas. n = 3 per group. ** P < 0.01, *** P < 0.001. ( g ) Spider graph representation (Log 10 scale) of multiplex analysis of MMPs in aortic extracts from WT or Ripk3 −/− mice. n = 3 ( Ripk3 −/− ) and 4 (WT). * P = 0.0454. Representative image of IF staining of MMP12, and its colocalization with CD68 macrophages in aortic section of mice treated as indicated ( h , i ). Scale bar = 20 µm. ( j ) Mmp12 mRNA in WT or Ripk3 −/− BMDM treated with HMGB1 (10 ng ml −1 ) in the presence or not of Mdivi-1 (10 µM) or MitoTEMPO (10 µM). n = 3 per group. * P < 0.05, ** P < 0.01. ( k ) Schematic representation of experimental protocol (top) and incidence of AAA in WT and Mmp12 −/− mice (below). i.n. intranasal. n = 4–5 per group. Representative color Doppler ultrasound images of aorta and M-mode screenshots (arrow) ( i ). Chronological quantifications of aortic diameter ( m ), maximal aortic diameter ( n ), and representative Verhoeff-Van Gieson staining in aortic sections of WT and Mmp12 −/− mice in the presence of ALI ( o ). n = 3 per group. Arrows indicate elastin breaks. Data is presented as mean, error bars represent s.e.m. (scale bar = 50 µm). * P = 0.0364. P va/clues were calculated by one-tailed ( g ) or two-tailed ( m , n ) unpaired t -tests, or one-way ANOVA ( a – f , j ).
Rabbit Anti Human Mmp12 Abx102901, supplied by Abbexa Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-human mmp12 abx102901/product/Abbexa Ltd
Average 90 stars, based on 1 article reviews
rabbit anti-human mmp12 abx102901 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
anti-mmp12 rabbit monoclonal antibody af1147  (Beyotime)
90
Beyotime anti-mmp12 rabbit monoclonal antibody af1147
MitoSOX quantification of WT and Ripk3 −/− BMDM treated with HMGB1 ( a ) and WT BMDM treated with HMGB1 in the presence or not of Mdivi-1 or MitoTEMPO ( b ). n = 3 . * P < 0.05, *** P < 0.001. ( c ) Relative mRNA expression of Glud1 in WT or Ripk3 −/− BMDM. n = 3 per group . * P < 0.05, *** P < 0.001. ( d ) Representative IF images of dihydroethidium (DHE) staining (red) of aortic sections of mice treated as indicated. Elastin autofluorescence (green, scale bar = 20 µm). ( e ) Heatmap representation of differentially expressed genes in macrophages expressing Ripk3 ( Ripk3 + ) or not ( Ripk3 − ). ( f ) qPCR analysis of <t>Mmp12</t> mRNA in WT or Ripk3 −/− aortas. n = 3 per group. ** P < 0.01, *** P < 0.001. ( g ) Spider graph representation (Log 10 scale) of multiplex analysis of MMPs in aortic extracts from WT or Ripk3 −/− mice. n = 3 ( Ripk3 −/− ) and 4 (WT). * P = 0.0454. Representative image of IF staining of MMP12, and its colocalization with CD68 macrophages in aortic section of mice treated as indicated ( h , i ). Scale bar = 20 µm. ( j ) Mmp12 mRNA in WT or Ripk3 −/− BMDM treated with HMGB1 (10 ng ml −1 ) in the presence or not of Mdivi-1 (10 µM) or MitoTEMPO (10 µM). n = 3 per group. * P < 0.05, ** P < 0.01. ( k ) Schematic representation of experimental protocol (top) and incidence of AAA in WT and Mmp12 −/− mice (below). i.n. intranasal. n = 4–5 per group. Representative color Doppler ultrasound images of aorta and M-mode screenshots (arrow) ( i ). Chronological quantifications of aortic diameter ( m ), maximal aortic diameter ( n ), and representative Verhoeff-Van Gieson staining in aortic sections of WT and Mmp12 −/− mice in the presence of ALI ( o ). n = 3 per group. Arrows indicate elastin breaks. Data is presented as mean, error bars represent s.e.m. (scale bar = 50 µm). * P = 0.0364. P va/clues were calculated by one-tailed ( g ) or two-tailed ( m , n ) unpaired t -tests, or one-way ANOVA ( a – f , j ).
Anti Mmp12 Rabbit Monoclonal Antibody Af1147, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-mmp12 rabbit monoclonal antibody af1147/product/Beyotime
Average 90 stars, based on 1 article reviews
anti-mmp12 rabbit monoclonal antibody af1147 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


MitoSOX quantification of WT and Ripk3 −/− BMDM treated with HMGB1 ( a ) and WT BMDM treated with HMGB1 in the presence or not of Mdivi-1 or MitoTEMPO ( b ). n = 3 . * P < 0.05, *** P < 0.001. ( c ) Relative mRNA expression of Glud1 in WT or Ripk3 −/− BMDM. n = 3 per group . * P < 0.05, *** P < 0.001. ( d ) Representative IF images of dihydroethidium (DHE) staining (red) of aortic sections of mice treated as indicated. Elastin autofluorescence (green, scale bar = 20 µm). ( e ) Heatmap representation of differentially expressed genes in macrophages expressing Ripk3 ( Ripk3 + ) or not ( Ripk3 − ). ( f ) qPCR analysis of Mmp12 mRNA in WT or Ripk3 −/− aortas. n = 3 per group. ** P < 0.01, *** P < 0.001. ( g ) Spider graph representation (Log 10 scale) of multiplex analysis of MMPs in aortic extracts from WT or Ripk3 −/− mice. n = 3 ( Ripk3 −/− ) and 4 (WT). * P = 0.0454. Representative image of IF staining of MMP12, and its colocalization with CD68 macrophages in aortic section of mice treated as indicated ( h , i ). Scale bar = 20 µm. ( j ) Mmp12 mRNA in WT or Ripk3 −/− BMDM treated with HMGB1 (10 ng ml −1 ) in the presence or not of Mdivi-1 (10 µM) or MitoTEMPO (10 µM). n = 3 per group. * P < 0.05, ** P < 0.01. ( k ) Schematic representation of experimental protocol (top) and incidence of AAA in WT and Mmp12 −/− mice (below). i.n. intranasal. n = 4–5 per group. Representative color Doppler ultrasound images of aorta and M-mode screenshots (arrow) ( i ). Chronological quantifications of aortic diameter ( m ), maximal aortic diameter ( n ), and representative Verhoeff-Van Gieson staining in aortic sections of WT and Mmp12 −/− mice in the presence of ALI ( o ). n = 3 per group. Arrows indicate elastin breaks. Data is presented as mean, error bars represent s.e.m. (scale bar = 50 µm). * P = 0.0364. P va/clues were calculated by one-tailed ( g ) or two-tailed ( m , n ) unpaired t -tests, or one-way ANOVA ( a – f , j ).

Journal: Nature Communications

Article Title: Lung-derived HMGB1 is detrimental for vascular remodeling of metabolically imbalanced arterial macrophages

doi: 10.1038/s41467-020-18088-2

Figure Lengend Snippet: MitoSOX quantification of WT and Ripk3 −/− BMDM treated with HMGB1 ( a ) and WT BMDM treated with HMGB1 in the presence or not of Mdivi-1 or MitoTEMPO ( b ). n = 3 . * P < 0.05, *** P < 0.001. ( c ) Relative mRNA expression of Glud1 in WT or Ripk3 −/− BMDM. n = 3 per group . * P < 0.05, *** P < 0.001. ( d ) Representative IF images of dihydroethidium (DHE) staining (red) of aortic sections of mice treated as indicated. Elastin autofluorescence (green, scale bar = 20 µm). ( e ) Heatmap representation of differentially expressed genes in macrophages expressing Ripk3 ( Ripk3 + ) or not ( Ripk3 − ). ( f ) qPCR analysis of Mmp12 mRNA in WT or Ripk3 −/− aortas. n = 3 per group. ** P < 0.01, *** P < 0.001. ( g ) Spider graph representation (Log 10 scale) of multiplex analysis of MMPs in aortic extracts from WT or Ripk3 −/− mice. n = 3 ( Ripk3 −/− ) and 4 (WT). * P = 0.0454. Representative image of IF staining of MMP12, and its colocalization with CD68 macrophages in aortic section of mice treated as indicated ( h , i ). Scale bar = 20 µm. ( j ) Mmp12 mRNA in WT or Ripk3 −/− BMDM treated with HMGB1 (10 ng ml −1 ) in the presence or not of Mdivi-1 (10 µM) or MitoTEMPO (10 µM). n = 3 per group. * P < 0.05, ** P < 0.01. ( k ) Schematic representation of experimental protocol (top) and incidence of AAA in WT and Mmp12 −/− mice (below). i.n. intranasal. n = 4–5 per group. Representative color Doppler ultrasound images of aorta and M-mode screenshots (arrow) ( i ). Chronological quantifications of aortic diameter ( m ), maximal aortic diameter ( n ), and representative Verhoeff-Van Gieson staining in aortic sections of WT and Mmp12 −/− mice in the presence of ALI ( o ). n = 3 per group. Arrows indicate elastin breaks. Data is presented as mean, error bars represent s.e.m. (scale bar = 50 µm). * P = 0.0364. P va/clues were calculated by one-tailed ( g ) or two-tailed ( m , n ) unpaired t -tests, or one-way ANOVA ( a – f , j ).

Article Snippet: Membranes were blocked and probed with: mouse anti-mouse GAPDH (ab8245, Abcam), rabbit anti-mouse β-actin (sc-130656, Santa Cruz Biotechnology), mouse anti-mouse HMGB1 (ab11354, Abcam), rabbit anti-mouse phosphoRIPK3 (ab195117, Abcam), rabbit anti-human phosphoRIPK3 (ab209384, Abcam), rabbit anti-mouse/human pDRP1 (Ser616, PA5-64821, Thermo Fisher Scientific), rabbit anti-mouse MMP12 (22989-1-AP, Proteintech) and rabbit anti-human MMP12 (abx102901, Abbexa) 1:1000 dilution each.

Techniques: Expressing, Staining, Multiplex Assay, One-tailed Test, Two Tailed Test

HMGB1 derived from injured lungs is captured by TLR4 transmural macrophages in the abdominal aorta. Activation of TLR4 by HMGB1 upregulates RIPK3 and activates its phosphorylation. RIPK3 activates phospho-DRP1 which triggers mitochondrial fission and increases mitochondrial reactive oxygen species (ROS) production. ROS stimulates the expression of MMP12 by macrophages responsible for elastin fiber degradation in the aorta. Inhibition of phosphorylation of RIPK3 on Serine 204 refrains DRP1 activation and subsequent MMP12 expression.

Journal: Nature Communications

Article Title: Lung-derived HMGB1 is detrimental for vascular remodeling of metabolically imbalanced arterial macrophages

doi: 10.1038/s41467-020-18088-2

Figure Lengend Snippet: HMGB1 derived from injured lungs is captured by TLR4 transmural macrophages in the abdominal aorta. Activation of TLR4 by HMGB1 upregulates RIPK3 and activates its phosphorylation. RIPK3 activates phospho-DRP1 which triggers mitochondrial fission and increases mitochondrial reactive oxygen species (ROS) production. ROS stimulates the expression of MMP12 by macrophages responsible for elastin fiber degradation in the aorta. Inhibition of phosphorylation of RIPK3 on Serine 204 refrains DRP1 activation and subsequent MMP12 expression.

Article Snippet: Membranes were blocked and probed with: mouse anti-mouse GAPDH (ab8245, Abcam), rabbit anti-mouse β-actin (sc-130656, Santa Cruz Biotechnology), mouse anti-mouse HMGB1 (ab11354, Abcam), rabbit anti-mouse phosphoRIPK3 (ab195117, Abcam), rabbit anti-human phosphoRIPK3 (ab209384, Abcam), rabbit anti-mouse/human pDRP1 (Ser616, PA5-64821, Thermo Fisher Scientific), rabbit anti-mouse MMP12 (22989-1-AP, Proteintech) and rabbit anti-human MMP12 (abx102901, Abbexa) 1:1000 dilution each.

Techniques: Derivative Assay, Activation Assay, Phospho-proteomics, Expressing, Inhibition